7.21.2019

How to make Biogenic ACP, Calcium Triphosphate - Biomimetic ACP

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So in one of my last posts (find the TEEF tag at the bottom of the site) I talked about a toothpaste or mouthwash that uses either biomimetic ACP or Octacalcium phosphate produced nano-hydroxyapatite.

In this post I will talk about what exactly the elusive calcium triphosphate is, how it is made, and how to then make biomimetic ACP (a pseudo biogenic ACP that we can easily make).

The biggest problem with this is that it requires a non metal blender.  Not very easy to find.  The reason for this is we will be blending an acidic solution at high speeds.  Acids quickly dissolve metal whereas alkaline solutions do not.  This fact alone makes this a far fetched idea and the octacalcium phosphate -> nano hydroxyapatite is likely a better solution for household or common lab equipment.  Nevertheless, lets proceed.  Using a glass beaker with a glass coated stirbar may be good enough.

Above is the molecule calcium triphosphate we are trying to create.  The way I propose to create it is to dissolve one mole of dicalcium phosphate with 2 moles of phosphoric acid.

CaHPO4 + 2(H3PO4) => Ca++ + 3(H2PO4)

So basically we have 1 calcium and 3 phosphates.  So in the above picture one of the phosphates needs to have 2 H's (the middle one with 2 bonds) and the other 2 need to have 1 H (it gives it up to bond with the middle phosphate in a condensation reaction).  Since the pKa of going from 2 H's to 1H for phosphoric acid is 7.2, we want a little more acidic than that, say ph 6.5-6.7 just as a rough guess.  The exact pH can be calculated from dissociation coefficients but I'm not brushed up on that calculation.  This guess is based on the pH of milk, a known source of biogenic ACP.

So my best hypothesis for creating this molecule is to use silica gel to help the condensation reaction proceed since we don't want to use strong acids or bases or noble metal catalysts.  This makes sense since silica is known to play a role in bone mineralization.

So my proposed process is to combine 1 mole of dicalcium phosphate with 2 moles phosphoric acid.  Blend or stir until fully dissolved in a small amount of water.  Having little water is important for the condensation reaction to proceed.  Next blend with solubilizing agents like amino acids/protiens, plant saponins, lecithin or other emulsifiers, and perhaps some fatty acids like lauric acid.  Start blending or stirring.  Slowly add calcium silicate until pH is between 6.5 and 6.7 (the pH of milk and also ACP).  The addition of the calcium during neutralization and the silica helping condensation should have not only created the calcium triphosphate but also converted the created calcium triphosphate to biomimetic ACP from the extra calcium.  This is why it is important for the whole process to be done while blending or stirring (in all glass).  The process of alkalizing to neutral pH slowly should cause the silica to cause the condensation reaction.  Not sure if heat would help but I would assume so.  The saponins, aminos, etc should help keep the calcium triphosphate formed in solution and on a nano-scale.

That's about it.  To sum it up:

1 mole Dicalcium phosphate

2 moles phosphoric acid

Amino's/saponins/Lecithin/fatty acids

Mix in glass

While mixing neutralize with calcium silicate until pH 6.5-6.7


In the following paper it appears: 0.498 g K2HPO4 was added into the CMC gel under stirring (500 rpm). Next, 0.555 g CaCl2 was added into 10 ml deionized water and this solution was added dropwise into the CMC gel under stirring for 5 min to form CMC/ACP gel

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294661/ 

So this needs to be investigated but it seems the gel is needed and the stirring is needed to form it which aligns with my process using silica gel.

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